Part:BBa_K2957020

TRE, IL-8 (CXCL8)

Contents: TRE, IL-8 (CXCL-8), NeonGreen tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for inducible IL-8 expression.

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL-8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 708
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 634
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 708
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 708
  Illegal AgeI site found at 714
  
• 1000
  COMPATIBLE WITH RFC[1000]